MediLumine has partnered with Labeo Technologies Inc. for distribution and applications development of the
LightTrack OiS200 modular in vivo imaging system for speckle, fluorescence, and intrinsic optical Imaging.
LightTrack OiS200 is a modular in vivo optical imaging system that removes the need for using many different imaging systems in vascular biology and neuroscience. The system can rapidly deliver data on hemodynamics, blood flow, optogenetic stimulation, and calcium signalling. It has been used in publications for functional hemoglobin imaging, imaging of blood flow changes, in-vivo optogenetics, and calcium signalling.
For calcium imaging, the LightTrack OiS200 system uses sequential LED based illumination (525 nm, 590 nm, and 625 nm) to obtain both calcium and intrinsic signals. These signals are then used to obtain the corrected calcium signal (ΔF/F, %) of each pixel. In the example image above, calcium imaging was performed with LightTrack OiS200 in head-fixed awake GCaMP6s adult mice before and after conditioning to understand long term plastic changes on cortical maps. ref. https://www.frontiersin.org/articles/10.3389/fncir.2020.00038/full
Cerebral Blood Flow and Hemodynamics
In this example, the LightTrack OiS200’s speckle imaging modality was used with 785 nm laser to image changes in blood flow and it’s intrinsic optical imaging modality was used for functional imaging of hemodynamics with intervals of 2 seconds and resolution at the micron scale. ref. https://www.frontiersin.org/articles/10.3389/fphys.2021.611984/full
In Vivo Optogenetics
In vivo optogenetics can be performed using the laser stimulation matrix available on LightTrack OiS200 to generate bihemispheric motor maps. In this image, Thy1-ChR2-YFP mice having received a photothrombotic stroke at postnatal day 7 underwent logitudinal optogenetic motor mapping both before and after 3 weeks of skilled forelimb training. Optogenetic cortical stimulation enabled imaging of motor function biomarkers and showed that perinatal stroke reduces motor map size and movement latency AUC in both hemispheres. ref. ref. https://www.jneurosci.org/content/41/28/6157
For vascular imaging, the LightTrack OiS200 uses the a 472 nm blue channel with the illumination and acquisition running at least 5 minutes. Other wavelengths are available upon request. In this example, fluorescent microbeads (FMOY-1.3, 460/594 nm, Cospheric, U.S.) were IV injected into C57/Bl6 mouse with scalp removed. The sum of all images was made in the analysis software showing the detailed anatomical structure of vasculature through the skull of the mouse. Publications
Movement correction method for laser speckle contrast imaging of cerebral blood flow in cranial windows in rodents. Guilbert, J., & Desjardins, M. (2022).
A Longitudinal Pilot Study on Cognition and Cerebral Hemodynamics in a Mouse Model of Preeclampsia Superimposed on Hypertension: Looking at Mothers and Their Offspring. Trigiani, L. J., Lecrux, C., Royea, J., Lavoie, J. L., Lesage, F., Pilote, L., & Hamel, E. (2021).
Developmental and interventional plasticity of motor maps after perinatal stroke. Zhang, S. Y., Jeffers, M. S., Lagace, D. C., Kirton, A., & Silasi, G. (2021).
Mesoscopic Mapping of Stimulus-Selective Response Plasticity in the Visual Pathways Modulated by the Cholinergic System. Laliberté, G., Othman, R., & Vaucher, E. (2020).
A Pilot Study Investigating Changes in Capillary Hemodynamics and Its Modulation by Exercise in the APP-PS1 Alzheimer Mouse Model. Lu, X., Moeini, M., Li, B., Lu, Y., Damseh, R., Pouliot, P., … & Lesage, F. (2019).
Correlation of hemodynamic and fluorescence signals under resting state conditions in mice’s barrel field cortex. Bélanger, S., Oliveira Ferreira de Souza, B., Casanova, C., & Lesage, F. (2016).