Vargulin

Description

Vargulin was first characterized by Dr. Osamu and Akemi Shimomura from “Umi Hotaru” (Japanese Sea Fireflies) in 1966. These bottom-dwelling ostracods emit a stream of light into the water when disturbed, presumably to deter predators. Vargulin’s corresponding luciferase was first cloned by Eric Thompson and S. Nagata under Fred Tsuji in Japan in 1989. Vargulin Luciferase is a 555 amino acid, 62.171 KDa protein that oxidizes the luciferin to produce blue light with an emission maximum at 462 nm. One advantage of using the Vargulin system is that it does not cross-react with Coelenterazine or Firefly d-Luciferin, making it ideal for multiplex assays and yielding brighter signals than Firefly Luciferase.

The advantages of lyophilized Vargulin include:

• Pre-aliquoted in small amounts, providing fresh substrate for every set of experiments
• Long shelf-life (packed under Argon)
• Faster dissolution due to its fine crystal structure
• Consistent quality between aliquots
  

The Vargulin is shipped as a filtered, lyophilized, batch-controlled substrate.

Properties

• Compound ID: Vargulin or Cypridina Luciferin
• Molecular Formula: C22H27N7O