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This water soluble coelenterazine has been designed for in vivo imaging of Renilla luciferase expression with peak light emission at around 480 nm. Scientists employ the Renilla luciferase reporter due to various factors such as its relatively low background signal, stability over long periods, and it’s compatibility with dual-reporter systems allowing researchers to monitor multiple parameters within the same experimental setup.
Morse D, Tannous BA. A water-soluble coelenterazine for sensitive in vivo imaging of coelenterate luciferases. Mol Ther. 2012 Apr;20(4):692-3. doi: 10.1038/mt.2012.38. PMID: 22472977; PMCID: PMC3321601.
Celinskis D, Black CJ, Murphy J, et al. Toward a brighter constellation: multiorgan neuroimaging of neural and vascular dynamics in the spinal cord and brain. Neurophotonics. 2024 Apr;11(2):024209. DOI: 10.1117/1.nph.11.2.024209. PMID: 38725801; PMCID: PMC11079446.
Adams PP, Flores Avile C, Jewett MW. A Dual Luciferase Reporter System for B. burgdorferi Measures Transcriptional Activity during Tick-Pathogen Interactions. Frontiers in Cellular and Infection Microbiology. 2017 ;7:225. DOI: 10.3389/fcimb.2017.00225. PMID: 28620587; PMCID: PMC5449462.
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Copyright © 2024 · MediLumine, Inc · All Rights Reserved