HREM 3D Episcopic Imaging System

Description

High-Resolution 3D Histological Imaging — Developed by Indigo Scientific, Distributed by MediLumine

The HREM 3D Episcopic Imaging System enables researchers to visualize tissue architecture in unprecedented detail. Using High-Resolution Episcopic Microscopy (HREM), this system acquires perfectly registered serial images directly from the blockface of resin-embedded specimens. The resulting datasets can be reconstructed into true 3D volumes without the distortions typically introduced by traditional section mounting or alignment.

This technology fills the gap between histology and microCT, allowing for micrometer-scale 3D visualization of complex biological structures such as embryos, organs, and vasculature.

Key Features

• High Resolution: Produces 1-8 micron complete voxel resolution over an entire volumetric dataset with micron resolution on samples up to 25 mm
• True 3D Histology: Captures inherently aligned image stacks with sub-cellular resolution.
• No tissue clearing
• Dense Tissues: Image dense tissue and bone at high contrast without variation
• Episcopic Illumination: Imaging of the blockface surface ensures perfect optical registration across slices.
• Versatile Contrast: Works with tissue autofluorescence or fluorescent dyes.
• Automated Sectioning: Integrated microtome provides consistent slice thickness (as low as 1 µm).
• Quantitative Analysis: Export image stacks for 3D reconstruction, segmentation, and morphometric analysis.
• Wide Range of Applications: Developmental biology, toxicological studies, tissue morphology, and phenotyping.

Technical Specifications