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PRISM IN VIVO IMAGING SYSTEM

Description

MediLumine’s EMCCD based PRISM™ uses photon counting for highly sensitive in vivo optical imaging of up to five mice. The imager is able to detect a single photon in a pixel opening a new biological window for bioluminescence and fluorescence imaging. In addition, the imaging system is sensitive at high acquisition speeds allowing for real-time imaging in bioluminescent and fluorescence modes. User’s of PRISM are finding that the system has an unparalleled combination of real-time imaging and detection of ultra-weak photonic emissions for deeper and earlier in vivo imaging. The design of PRISM is completely modular allowing scientists to purchase either base Bioluminescent imager or Bioluminescent and Epi-Fluorescent System or Bioluminescent with Epi- and Trans-Fluorescent modules for NIR fluorescent 3D imaging.

Application Examples

Tumor cells were injected i.v. and time-lapse in vivo images of lung and liver tumor growth were detected by luminescence (luciferase) in mice without immune reconstitution (no-AT), injected i.v. with 1 × 106 MSCs following Auto-AT of 1 × 107 WBC (Auto-AT) or with AT of WBC and the injection of MSCs (Auto-AT plus MSC). Also shown are images of tumors by fluorescence (mPlum) at sacrifice. ref. https://www.jimmunol.org/content/203/10/2735

Publications

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KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors

Mac Donald, A., Guipouy, D., Lemieux, W., Harvey, M., Bordeleau, L. J., Guay, D., … & Haddad, E. (2023). KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors. Frontiers in Immunology, 14.

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Fluorescent pH-sensing bandage for point-of-care wound diagnostics

Al-Hawat, M. L., Tricou, L. P., Cherifi, K., Lamontagne, S., Tran, M., Ching Yie Ngu, A., … & Matoori, S. (2023). Fluorescent pH-sensing bandage for point-of-care wound diagnostics. bioRxiv, 2023-10.

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CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling

Serwe, G., Kachaner, D., Gagnon, J., Plutoni, C., Lajoie, D., Duramé, E., … & Therrien, M. (2023). CNK2 promotes cancer cell motility by mediating ARF6 activation downstream of AXL signalling. Nature Communications, 14(1), 3560.

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Repurposing disulfiram, an alcohol-abuse drug, in neuroblastoma causes KAT2A downregulation and in vivo activity with a water/oil emulsion

Beaudry, A., Jacques-Ricard, S., Darracq, A., Sgarioto, N., Garcia, A., García, T. R., … & Raynal, N. J. M. (2023). Repurposing disulfiram, an alcohol-abuse drug, in neuroblastoma causes KAT2A downregulation and in vivo activity with a water/oil emulsion. Scientific Reports, 13(1), 16443.

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Therapy-induced senescence drives immunotherapy resistance by inducing an immunosuppressive tumor microenvironment.

Beauséjour, C., Maggiorani, D., Le, O., Lisi, V., Landais, S., Moquin-Beaudry, G., … & Decaluwe, H. (2023). Therapy-induced senescence drives immunotherapy resistance by inducing an immunosuppressive tumor microenvironment.

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Nuclear Location Bias of HCAR1 Drives Cancer Malignancy through Numerous Routes

Nezhady, M. A. M., Cagnone, G., Bajon, E., Chaudhari, P., Modaresinejad, M., Hardy, P., … & Chemtob, S. (2022). Nuclear Location Bias of HCAR1 Drives Cancer Malignancy through Numerous Routes. bioRxiv, 2022-07.

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Optimization of a liposomal DNase I formulation with an extended circulating half-life

Penaloza Arias, L. C., Huynh, D. N., Babity, S., Marleau, S., & Brambilla, D. (2022). Optimization of a liposomal DNase I formulation with an extended circulating half-life. Molecular Pharmaceutics, 19(6), 1906-1916.

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Restored immune cell functions upon clearance of senescence in the irradiated splenic environment

Palacio, L., Goyer, M. L., Maggiorani, D., Espinosa, A., Villeneuve, N., Bourbonnais, S., … & Beauséjour, C. (2019). Restored immune cell functions upon clearance of senescence in the irradiated splenic environment. Aging Cell, 18(4), e12971.

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The tumor immune response is not compromised by mesenchymal stromal cells in humanized mice

Moquin-Beaudry, G., Colas, C., Li, Y., Bazin, R., Guimond, J. V., Haddad, E., & Beauséjour, C. (2019). The tumor-immune response is not compromised by mesenchymal stromal cells in humanized mice. The Journal of Immunology, 203(10), 2735-2745.

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INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice

Le, O., Palacio, L., Bernier, G., Batinic-Haberle, I., Hickson, G., & Beauséjour, C. (2018). INK4a/ARF expression impairs neurogenesis in the brain of irradiated mice. Stem cell reports, 10(6), 1721-1733.

Development of a new bioactivatable fluorescent probe for quantification of apolipoprotein A-I proteolytic degradation in vitro and in vivo

Maafi, F., Li, B., Gebhard, C., Brodeur, M. R., Nachar, W., Villeneuve, L., … & Tardif, J. C. (2017). Development of a new bioactivatable fluorescent probe for quantification of apolipoprotein AI proteolytic degradation in vitro and in vivo. Atherosclerosis, 258, 8-19.

Impact of hMSCs on solid tumor growth in novel tumor/immune system humanized mice

Moquin-Beaudry, G., Desaulniers-Langevin, C., Benabdallah, B., Palacio, L., Beauséjour, C. (2017). Virtual Keystone Symposia.

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In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells – Cancer Immunology, Immunotherapy

Díaz-Rodríguez, Y., Cordeiro, P., Belounis, A., Herblot, S., & Duval, M. (2017). In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells. Cancer Immunology, Immunotherapy, 66(10), 1307-1320.

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Hybrid FMT-MRI applied to in vivo atherosclerosis imaging

Li, B., Maafi, F., Berti, R., Pouliot, P., Rhéaume, E., Tardif, J. C., & Lesage, F. (2014). Hybrid FMT-MRI applied to in vivo atherosclerosis imaging. Biomedical optics express, 5(5), 1664-1676.

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MRI-guided fiber-based fluorescence molecular tomography for preclinical atherosclerosis imaging

Li, B., Pouliot, P., & Lesage, F. (2014, September). MRI-guided fiber-based fluorescence molecular tomography for preclinical atherosclerosis imaging. In Photonics North 2014 (Vol. 9288, pp. 184-189). SPIE.

Generation of Functional Human T Cell Development in NOD/SCID/IL2rγnull Humanized Mice Without Using Fetal Tissue: Application as a Model of HIV Infection and Persistence

Colas, C., Volodina, O., Beland, K., Pham, T. N., Li, Y., Dallaire, F., … & Haddad, E. (2022). Generation of Functional Human T Cell Development in NOD/SCID/IL2rγnull Humanized Mice Without Using Fetal Tissue: Application as a Model of HIV Infection and Persistence. bioRxiv.

Poster

Colas C, Li Y, Dicaire R, Guimond J, Patey N, Haddad E. Optimization of the humanized NOD/SCID/IL2rγnull Bone marrow-Liver-Thymus (BLT) mouse model. 5th International Workshop on Humanized Mice, January 28-30, 2016 Zurich, Switzerland

Poster

Colamartino A, Nicoletti S, Herblot S, Duval M, Haddad E. New approach in immunotherapy of acute lymphoblastic leukemia. 29th Annual Spring Meeting of the Canadian Society for Immunology, April 1–4, 2016 Ottawa, ON, Canada

Poster

Gaël Moquin-Beaudry, Cynthia Desaulniers-Langevin, Basma Benabdallah, Lina Palacio, Christian Beauséjour. IMPACT OF hMSCs ON SOLID TUMOR GROWTH IN NOVEL TUMOR/IMMUNE SYSTEM HUMANIZED MICE, 2017 Keystone Symposium: Cancer Immunology and Immunotherapy: Taking a Place in Mainstream Oncology (C7), March 19-23 2017, Whistler, BC, Canada.

Poster

Gaël Moquin-Beaudry, Cynthia Desaulniers-Langevin, Basma Benabdallah, Lina Palacio, Christian Beauséjour. IMPACT OF hMSCs ON SOLID TUMOR GROWTH IN NOVEL TUMOR/IMMUNE SYSTEM HUMANIZED MICE, 2016 ISSCR meeting, June 22-25 2016, San Francisco, CA, United States.

Poster

Gaël Moquin-Beaudry, Lina Palacio, Pascal Bardeau, Basma Benabdallah, Oanh Le, Christian M. Beauséjour. Development and applications for a novel human autologous tumor/immune system xenograft model in mice, 2015 Canadian Cancer Research Conference, November 8-10 2015, Montreal, QC, Canada.

Poster

Gaël Moquin-Beaudry, Lina Palacio, Basma Benadballah, Christian Beauséjour. Impact of hUC-MSCs on tumor growth in a humanized autologous tumor/immune system xenograft model in mice, 2015 Montreal Immunology Meeting, November 23 2015, Montreal, QC, Canada.

Poster

Lina Palacio, Oanh Lee, Gaël Moquin-Beaudry, Sara Bourbonnais, Christian Beausejour. IR-induced p16INK4a expression in the splenic niche modulates the immune system. XXXIe Congrès des étudiants gradués et post-gradués du centre de recherche du CHU Ste-Justine, 2016, Montreal, Canada.

Poster

Lina Palacio, Oanh Lee, Gaël Moquin-Beaudry, Sara Bourbonnais, Christian Beausejour. IR-induced p16INK4a expression in the splenic niche modulates the immune system. XXI Journée de la recherche Gabriel L. Plaa, 2016, Montreal, Canada.

Poster

Lina Palacio, Oanh Lee, Christian Beausejour. IR-induced p16INK4a expression in the spleen impairs immune cell functions,Montreal Immunology Meeting (MIM), 2015, Montreal, Canada.

Poster

Lina Palacio, Oanh Lee, Christian Beausejour. L’expression du gène suppresseur de tumeur p16INK4a dans le microenvironnement tumoral joue en rôle important dans la réponse thérapeutique chez les souris. XX Journée de la recherche Gabriel L. Plaa, 2015, Montreal, Canada.

Poster

Lina Palacio, Oanh Lee, Christian Beausejour. L’expression du gène suppresseur de tumeur p16INK4a dans le microenvironnement tumoral joue en rôle important dans la réponse thérapeutique chez les souris. XXX congrès des étudiants gradués et post- gradués du centre de recherche du CHU Ste-Justine, 2015, Montreal, Canada.

Poster

L. Palacio, S. Bourbonnais, N. Villeneuve, G. Moquin-Beaudry, O. Le, H. Decaluwe, C. Beauséjour. IR-induced senescence of the splenic microenvironment impacts murine immune cells functions, International Cell Senescence Association (ICSA) Conference 2017, Paris, France.

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