The OTF7000 Cryostat is engineered to provide highly reproducible frozen tissue sections with exceptional precision and stable temperature control, making it an essential instrument for high-throughput laboratories in clinical, research, and industrial settings. Available in two configurations, the semi-automated OTF7000 and the fully automated OTF7000/A, which features integrated motorised sectioning, both models are designed to deliver consistent section quality and incorporate advanced features to meet the most stringent cryosectioning requirements.
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The OTF7000 Cryostat delivers the precision and reproducibility critical for a broad spectrum of advanced research and clinical applications. In Clinical Histology and Pathology, it is indispensable for accurate intraoperative frozen section analysis, supporting tumor profiling, metastasis detection, and the assessment of treatment efficacy. For Immunohistochemistry, the instrument facilitates protein localization studies and the comprehensive analysis of cellular markers, antigens, and disease-specific biomarkers.
In Developmental Biology, this cryostat enables detailed investigations into organogenesis and cellular differentiation, supports stem cell and tissue engineering research, and allows for precise molecular tissue composition analysis across various developmental stages. Researchers in Pharmacology and Toxicology utilize the OTF7000 Cryostat for the evaluation of therapeutic outcomes, alongside critical pharmacokinetic and biodistribution studies. Furthermore, it plays a vital role in Immunology and Infectious Disease research for understanding pathogen and immune cell distribution, analyzing inflammation responses, and investigating autoimmune disease mechanisms.
The precision afforded by the OTF7000 Cryostat is also crucial for Spatial Transcriptomics, where optimal cryosectioning ensures high-quality data acquisition for mapping gene expression within complex tissue architectures at high spatial resolution. Its capabilities extend to various non-infectious disease research areas, including Neurology, Cardiology, and Oncology, supporting advanced studies of disease progression, pathological features, cellular and molecular changes, and gene expression mechanisms.
The OTF7000 Cryostat ensures precise and stable operation through a suite of integrated engineering features. High precision temperature control is maintained within the cryochamber, adjustable from 0 to –35°C, supported by a variable speed compressor for consistent cooling. A dedicated sample cooling system provides an adjustable range from –10°C to –50°C via Peltier cooling, complemented by a cryobar sample preparation station capable of reaching –60°C for rapid tissue freezing. An intelligent temperature control system offers precise regulation for both individually controlled specimen holder and chamber temperatures.
Stable and accurate sectioning is achieved through advanced mechanical design. The system incorporates precision cross roller guide rails for exceptionally smooth operation, screw rods meeting 0.5 µm sample injection requirements, and a five-phase, high-resolution stepping motor that minimizes vibration and ensures precise positioning. The horizontal sampling slide is crafted from corrosion-resistant, aviation-grade aluminium to maintain long-term performance and reliability. The OTF7000/A model further enhances reproducibility and reduces operator variability with its integrated motorised sectioning capability.
Operational efficiency is prioritized with a 7-inch touch-sensitive screen for intuitive control and joystick control for seamless trimming, coarse sampling, and section switching. A ±8° visual pointer assists in precise specimen clamp positioning, thereby reducing trimming time and minimizing sample waste. An auto-sectioning mode ensures consistent results, while multiple defrosting options and an automatic sleep/wake function contribute to energy savings. Specimen loading and marking are simplified with a single-lever system for easy sample identification and workflow optimization.
Integrated safety features include a low-temperature UVC disinfection system, demonstrably effective against bacteria and viruses at intensities above 340 µW/cm², enhancing user safety when processing biological specimens. The ergonomic cabinet features a circular arc design, with an optional height-adjustable footrest available for operator comfort during extended use. For laboratory cleanliness and convenience, an optional section waste removal system minimizes manual cleaning, supported by a waste management system that provides a real-time display of liquid volume and alarm notifications when the container reaches capacity. The cryostat also supports a wide range of compatible accessories to further optimize laboratory workflow and versatility.
